Sera randomly selected from healthy young adults were titrated in indirect immunofluorescence test for antibodies to the Epstein Barr virus (EBV).
B95-8 cell line was used as antigen and Raji cell line as negative control. These cells were smeared on slide glass after culture on RPM! 1640 with 10% fetal bovine serum.
The last serum dilution-yielding detectable fluorescence elicited by a few scattered cells which could be distinguished from the nonspecific surrounding cells-was taken as the endpoint of positivity. The results obtained were as follows:
1. The incidence of positive sera was 97% in young adults, 67% in children and 93% in neonates.
2. Geometric mean titers of adults, children and neonates were 1:45.7, 1:13.1, 1:22.8, respectively. 3. High titers (> 1:160) in adults, children and neonates were 16%, 8%, 7% respectively.
From above, nearly all Korean adults have antibodies to the Epstein-Barr virus. The results of female group were relatively similar to those of neonate group. And the results suggest that the primary infection was occurred relatively high rate at childhood.
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